Please use this identifier to cite or link to this item: https://rep.polessu.by/handle/123456789/16967
Title: Lys-plasminogen stimulates vitronectin exposure on the platelet surface
Authors: Zhernossekov, D.D.
Roka-Moiia, Y.M.
Tykhomyrov, A.A.
Grinenko, T.V.
Keywords: plasminogen
platelets
platelet secretion
vitronectin
flow cytometry
Issue Date: 2015
Citation: Lys-plasminogen stimulates vitronectin exposure on the platelet surface / D.D. Zhernossekov [и др.] // Biopolimers and Cells. – 2015. – Vol. 31, Number 2. – P. 104-108.
Abstract: Aim.To study the vitronectin exposure on the platelet surface in the presence of two forms of the plas-minogen molecule: Lys- and Glu-plasminogens. Methods.Research was conducted on human platelets. Aggregometry was used to check the platelet vitality and cell response towards thrombin. To evaluate the influence of different plasminogen forms on the platelet vitronectin exposure we used the method of flow cytometry. Results. It was found that incubation of resting platelets with Lys-plasminogen increased the amount of vitronectin-positive cells, but did not affect significantly their fluorescent intensity. Thrombin stimulation led to an increase in both: the number of vitronectin-positive platelets and the signal of fluorescence at least by two times. The Lys-plasminogen adding to the suspension of washed platelets followed by the thrombin stimulation enhanced the vitronectin exposure on the platelet surface and increased the amount of vitronectin-positive cells as compared to the isolated thrombin stimulation. Glu-plasminogen had no effect on the vitronectin exposure in case of resting or stimulated platelets. Conclusions. Lys-plasminogen but not its Glu-form enhances the exposure of vitronectin on the platelet surface. We suggest that the binding of Lys-plasminogen to the surface platelet receptors may generate plasmin that leads to the activation of intracellular signaling cascade.
Appears in Collections:Публикации сотрудников / Publications of the teaching stuff of Polessky State University

Files in This Item:
File Description SizeFormat 
Zhernossekov_DD_Lys-plasminogen_stimulates_vitronectin_exposure_on_the_platelet_surface.pdf256.77 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.