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Title: | Surface-exposed actin binds plasminogen on the membrane of agonist-activated platelets: a flow cytometry study |
Authors: | Tykhomyrov, A.A. Zhernossekov, D.D. Grinenko, T.V. |
Keywords: | platelets surface-exposed actin plasminogen flow cytometry |
Issue Date: | 2017 |
Citation: | Tykhomyrov, A.A. Surface-exposed actin binds plasminogen on the membrane of agonist-activated platelets: a flow cytometry study / A. O. Tykhomyrov, D.D. Zhernossekov, T. V. Grinenko // Biopolymers and Cell. – 2017. – 33, № 3. – P. 172-182. |
Abstract: | Aim.To elucidate whether actin exposed on the surface of agonist-activated platelets is re-sponsible for plasminogen binding. Methods. Human washed platelets were obtained by gel-filtration on Sepharose-2B. The levels of exposed actin on the outer surface of plasma membrane of intact and thrombin- or collagen-stimulated platelets were monitored by flow cytometry. The binding of plasminogen fluorescent conjugate (Pg-FITC) with platelets treated with anti-actin antibody or non-immune IgG (control) was analyzed cytometrically. Results. Thrombin or collagen exposure resulted in the dose-dependent actin expression on the surface of stimulated platelets. Pre-treatment of stimulated platelets with specific anti-actin antibodies was shown to significantly prevent the binding of Pg-FITC with the platelet surface. Conclusion. The results of the present study indicate that actin exposed on the platelet surface at the agonist-induced activation is involved in the plasminogen binding. |
Appears in Collections: | Публикации сотрудников / Publications of the teaching stuff of Polessky State University |
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Tykhomyrov_AA_Surface-exposed_actin_binds_plasminogen_on_the_membrane_of_agonist-activated_platelets.pdf | 739.76 kB | Adobe PDF | View/Open |
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