Эпигенетическая топография лимфоцитов у лиц, подвегшихся низкоуровневому ионизирующему воздействию

Abstract

The prior investigations have shown the increase of thrombosis risk in atherosclerosis in patients, chronically affected by low-level radiation. This was related to platelet hemostasis activation, hypercoagulable state, hemorheologic abnormalities (lower red blood cells flexibility, higher aggregation and increase of whole blood and/or plasma viscosity at high and low shear rates). These hemostaseologic and rtieologic features were independent from atherosclerotic localization, reflected the systemic character of atherosclerotic process. Object of research 10 patients were «liquidators» of Accidenton Chernobyl Nuclear Power Plant in 1986, 7 patients - inhabitants from territories, contaminated i37Cs with level more 5 Ku/km2 (a main group). Other 10 patients without additional low-level radiation exposure comprised a control group. The method of morphodensitometry gives the possibility to study different cytoiogical objects on a new quality level. Computational analysis of the TV image was performed by means of «DiaMorph» (Russia) - system combined with the microscopes «ZEISS» (Genvany). The computer image processing reveals an inside stnicture of the object which allows to research different type of cells on supramolecular level. We used a median filtration, linear median contrast, gauss transformation for analysis of topology supramolecular structure of chromatin. The chromatin network was recovered as a graphical drawing that connected maximal optical density on single vectors of TV scans. Analysis of an interphase chromatin in patients with an atherosclerosis has shown, that nucleus of lymphocytes at patients with in atherosclerosis have smaller perimeter (p <0.05) and optical density (p <0.05). Differences were found in patients of the basic group in heterochromatin areas of nucleus of lymphocytes: decrease of granular components dispersity, but increase of its contrast; decrease of the general area and perimeter of perigranular zone. Changes of the parameters describing a state of perigranular zone, is a marker of structurally functional rearrangements of a chromatin of the lymphocytes connected with in low-level irradiation.