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Title:	О возможности участия активных форм кислорода в расщеплении нуклеиновых кислот нуклеазами
Other Titles:	On the possibility of participaton of active oxygen species in a cleavage of nucleic acids by nucleases
Authors:	Пыжова, Н.С. Никандров, В.Н. Pyzhova, N.S. Nikandrov, V.N.
Keywords:	active oxygen species DNA RNA nuclease
Issue Date:	2009
Citation:	Пыжова, Н.С. О возможности участия активных форм кислорода в расщеплении нуклеиновых кислот нуклеазами / Н.С. Пыжова, В.Н. Никандров // Новости медико-биологических наук : научно-практический и научно-теоретический журнал. – 2009. – № 4. – С. 57-62.
Description:	Effect of scavengers of various active oxygen species on DNA or RNA cleavage by pancreatic DNAase or RNAase accordingly was studied. For the first time the inhibition of nucleases’ activity by the scavenger of superoxide radical – nitrotetrazolium blue in the presence of 8 M (but not 4 or 7 М) urea was demonstrated. It is supposed, that the effect was caused by loosening of nucleases’ globules and increase of availability of nuclease radical-generating sites for the scavenger. The reasons of inaffectiveness of superoxide radical scavengers such as superoxide dismutase or streptokinase on nucleicacids’ cleavage by the nucleases are discussed. For the first time the inhibition of nucleases’ enzymic activity was showed in the absence of urea, but under saturation of the system with nitrotetrazolium blue. It gives the base to consider the substrate – nucleic acid as an independent source of superoxide radicals, which are functionally significant for nuclease catalysis. Set of the obtained data allows to consider the function of nucleases as mediated with intire superoxide radicals, produced by "enzymesubstrate" system.
Appears in Collections:	Публикации сотрудников / Publications of the teaching stuff of Polessky State University

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