Взаимодействие нуклеотидов с супероксидными радикалами в модельных системах

Abstract

In the systems of generation (methionine-riboflavin, NADH-phenazine methosulfate, ascorbate-phenazine methosulfate) the reduction of nirtoblue tetrazolium is inhibited by ATP or GTP at the final concentration of 10~ 3 M (but not by UTP, CTP, ADP, AMP, inorganic pyrophosphate, riboso-5-phosphate). This effect was dependent on the composition of the generation systems but independent of the presence of iron in the nucleotides. It is suggested that ATP and GTP modifications may be ОД (or oxigen)-dependent which is determined by the conformation of nucleotide molecules.