Динамика белков культуральной жидкости при росте бета-гемолитических стрептококков группы С

Abstract

The dynamic study of the protein spectrum of culture fluid during the growth of p-hemolytic streptococcal strain H46A has been carried out by the methods of electrophoresis and isoelectrofocussing in polyacrylamide gel. Changes in the protein spectrum have phasic character and, on the whole, reflect the state of the microbial population, the presence of fractions corresponding to streptokinase and streptolysins being detected at all phases of growth. The electrophoretic mobility of streptokinase perceptibly changes at the end of the logarithmic phase; as shown by electrofocussing, at all stages of the population growth the heterogeneity of streptokinase is observed.