Identification new potential multidrug resistance proteins of Saccharomyces cerevisiae

Abstract

ABC (ATP-binding cassette) proteins can transport metabolic molecules and removes metabolic products and xenobiotics from the cell. The important problem is to study activity and search inhibitors of ABC proteins. There is a problem that ABC-proteins can transport hydrophobic drugs across the cell membrane due to their high substrate specificity. According to published data, Saccharomyces cerevisiae is an ideal model organism for analysis a lot of functional processes and gene activities of human cells. The aim of the present work is to reveal new potential yeast MDR proteins in S. cerevisiae with novel approach based on the cluster analysis. According to the cluster analysis of yeast ABCB subfamily, STE6 protein is turned out to be the most related to human P-gp protein. The largest number of homologues with human MDR proteins was found in the yeast ABCC subfamily. Yeast BPT1 and YCF1 proteins are shown to be the most phylogenetically close to human MRP1. In the ABCG subfamily of yeast, PDR10, PDR12, PDR15 and PDR18 are turned out to be potential proteins of multidrug resistance. The future experimental study of these subfamilies should be conducted in order to confirm the role of STE6, YCF1, BPT1, PDR10, PDR12, PDR15 and PDR18 in MDR phenotype of yeast and to study their activity modulators.