Please use this identifier to cite or link to this item: https://rep.polessu.by/handle/123456789/16964
Title: Study of the sites of plasminogen molecule which are responsible for inhibitory effect of Lys-plasminogen on platelet aggregation
Authors: Roka-Moya, Y.M.
Zhernossekov, D.D.
Yusova, E.I.
Kapustianenko, L.G.
Grinenko, T.V.
Keywords: plasminogen kringles
platelet aggregation
platelet plasminogen receptors
Lys-plasminogen
actin
Issue Date: 2014
Citation: Study of the sites of plasminogen molecule which are responsible for inhibitory effect of Lys-plasminogen on platelet aggregation / Y.M. Roka-Moya [и др.] // Ukrainian Biochemical Journal. – 2014. – Vol. 86, № 5. – P. 82-88.
Abstract: Plasminogen/plasmin system is involved in such important processes as thrombosis, inflammation and cancer. plasmin and plasminogen mediate their action through plasminogen-binding proteins on the cell surface. Lys-plasminogen, but not Glu-plasminogen, shows inhibitory effect on platelet aggregation induced by aDp, collagen and thrombin in preparations of both: platelet-rich plasma and washed platelets. We have shown that the kringle domains of Lys-plasminogen mediate interaction of this proenzyme with platelet-surface proteins. the aim of the work is to study the role of certain kringle domains in the inhibitory effect of Lys-plasminogen and to determine possible plasminogen-binding proteins on the platelet surface. all studied plasminogen fragments (k1-3, k4 and k5) abolished the inhibitory effect of Lys-plasminogen on platelet aggregation. We observed that k5 was more effective than k1-3 and k4. Biotin-labeled Lys-plasminogen, Glu-plasminogen and plasminogen fragment K1-3 possessed the highest affinity for actin, whereas the binding of biotin-labeled mini-plasminogen and k4 to actin was negligible. We have suggested that inhibitory effect of Lys-plasminogen is due to the interaction of kringle domains of this proenzyme with membrane-bound proteins which are exposed on the platelet surface during activation and are involved in thrombus formation.
URI: https://rep.polessu.by/handle/123456789/16964
Appears in Collections:Публикации сотрудников / Publications of the teaching stuff of Polessky State University



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