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Title: | Study of the sites of plasminogen molecule which are responsible for inhibitory effect of Lys-plasminogen on platelet aggregation |
Authors: | Roka-Moya, Y.M. Zhernossekov, D.D. Yusova, E.I. Kapustianenko, L.G. Grinenko, T.V. |
Keywords: | plasminogen kringles platelet aggregation platelet plasminogen receptors Lys-plasminogen actin |
Issue Date: | 2014 |
Citation: | Study of the sites of plasminogen molecule which are responsible for inhibitory effect of Lys-plasminogen on platelet aggregation / Y.M. Roka-Moya [и др.] // Ukrainian Biochemical Journal. – 2014. – Vol. 86, № 5. – P. 82-88. |
Abstract: | Plasminogen/plasmin system is involved in such important processes as thrombosis, inflammation and cancer. plasmin and plasminogen mediate their action through plasminogen-binding proteins on the cell surface. Lys-plasminogen, but not Glu-plasminogen, shows inhibitory effect on platelet aggregation induced by aDp, collagen and thrombin in preparations of both: platelet-rich plasma and washed platelets. We have shown that the kringle domains of Lys-plasminogen mediate interaction of this proenzyme with platelet-surface proteins. the aim of the work is to study the role of certain kringle domains in the inhibitory effect of Lys-plasminogen and to determine possible plasminogen-binding proteins on the platelet surface. all studied plasminogen fragments (k1-3, k4 and k5) abolished the inhibitory effect of Lys-plasminogen on platelet aggregation. We observed that k5 was more effective than k1-3 and k4. Biotin-labeled Lys-plasminogen, Glu-plasminogen and plasminogen fragment K1-3 possessed the highest affinity for actin, whereas the binding of biotin-labeled mini-plasminogen and k4 to actin was negligible. We have suggested that inhibitory effect of Lys-plasminogen is due to the interaction of kringle domains of this proenzyme with membrane-bound proteins which are exposed on the platelet surface during activation and are involved in thrombus formation. |
Appears in Collections: | Публикации сотрудников / Publications of the teaching stuff of Polessky State University |
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