Аб стане астаткаў тыразіну і трыптафану ў малекуле стрэптакіназы

Abstract

The spectral luminescent properties of streptokinase .have been studied. All the tyrosine residues are found to be ionized within one stage with ionization constant of 10.7. The state and availability of tryptophane residues have been investigated by the fluorescence method in a wide range of pH (1—12) with addition of different extinguishers! (Cs+, N0^, acrylamide) and solvents of different polarities (dioxane, glycerol). Trypto4 phane residues are shown to be localized at postively charged sites of the globule, per-; haps, in the «pockets» of the streptokinase molecule surface.