Оценка уровня экспрессии и каталитической активности изоэнзимов цитохрома P450 в опухолевой клеточной линии А549

Abstract

The contribution of monooxygenase component in carcinogenic activation of a key procarcinogenic derivative of benzo(a)pyrene – 7,8-benzo(a)pyrenediol in lung adenocarcinoma cell line A549 has been evaluated. It has been shown that the contribution of monooxygenase process under the experimental conditions in A549 cells is only 13 %, which corresponds with the relatively low level of constitutive expression of CYP1A1 and CYP1B1. When cells are exposed to 20-methylcholanthrene, monooxygenase component in carcinogenic activation of 7,8-benzo(a)pyrenediol has reached 25 %. Moreover, 90 % is accounted for a “complete” carcinogen – diolepoxide-2. As 20-methylcholanthrene is a polycyclic aromatic compound and exists with a benzo(a)pyrene in the tobacco smoke, this suggests a whole pool of polycyclic aromatic hydrocarbons entering the organism may significantly affect not only the level but also the direction of carcinogenic PAH activation of individual compounds.